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Testosterone Test

Serum testosterone test, Total testosterone

  • Summary
    The testosterone test determines the amount of testosterone in the blood, helping assess reproductive health, puberty, or hormonal imbalances in both man and women. This test is done by taking a blood sample from a vein in your arm, and fasting is required for this test for accurate results.Read more
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    Blood
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  • AgeAll Age Group
  • GenderMale and Female
  • FastingNot Required
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Know More About The Test

Dr. Viraj Sadrani
Medically Reviewed By Dr. Viraj Sadrani, MBBS, MD (Pathology)
Written By Dr. Nikita Toshi, BDS (Bachelor of Dental Surgery)
10 min read • Last Updated On: 28 January 2026 | 10:55 AM (IST)
A quick info on Testosterone Test

A quick info on Testosterone Test

Overview

Overview

The testosterone test determines the amount of testosterone in the blood. When the testosterone levels are too high or low, both men and women experience health issues.

Testosterone is a type of sex hormone that plays a vital role in both men and women. It controls sex drive, bone mass, muscular mass and sperm count in men. In women, ovaries generate testosterone in small amounts to help with growth and maintenance. Testosterone is responsible for hormone balance and other bodily processes.

Male gonads produce large amounts of testosterone during puberty. Testosterone affects secondary sexual traits such as muscle development, beard, moustache, chest hair, voice deepening and increased genital size. In men, decreased levels of testosterone mean decreased libido.

Clear causes for testosterone elevation in the blood are still under research. However, genetic diseases such as congenital adrenal hyperplasia, cancers of the adrenal, testicular and ovarian tumours cause a spike in testosterone values. Excessive use of testosterone supplements is one of the most common causes of high testosterone.

In women, high testosterone means increased masculine traits. In women, abnormal testosterone levels can be distressing. Excess testosterone in women causes hirsutism, acne, menstruation issues and infertility. Females may notice symptoms like loss of libido or mood changes as testosterone levels decline.

Low testosterone levels in men can cause partial or total hypogonadism. In hypogonadism, there will be changes in male secondary sexual characteristics and reproductive functions. In men, low testosterone levels create few or no symptoms.

Risk Assessment

Risk Assessment

Sexual disorders, PCOS, Infertility, Hypogonadism, Testicular cancer

What does this Test Detect?

What does this Test Detect?

The testosterone test measures the level of testosterone in the blood in both men and women.

In general, for adult men, a free-testosterone test is recommended if there are symptoms of low testosterone. In women, a testosterone test is recommended if there are symptoms of high testosterone levels.

Some disease conditions in which testosterone values are measured are:

  • Infertility
  • Hypogonadism
  • Testicular failure
  • Tumours of ovaries or testes
  • Osteoporosis
  • PCOS in women
  • Tumour of the adrenal gland
  • Congenital adrenal hyperplasia

The doctor recommends the testosterone test when an individual is suffering from a low sexual drive or suffering from symptoms of hormonal disturbance.

Indications for Testosterone Test

Indications for Testosterone Test

Some of the common symptoms of low testosterone range in men are:

  • Low sexual drive
  • Low libido
  • Lack of masculine muscles
  • Early ejaculation
  • Erectile dysfunction
  • Enlarged breasts
  • Lack of energy
  • Easy fatigue
  • Trouble with concentration
  • Hair loss
  • Narrow shoulders

In females, a testosterone test is used for the diagnosis of causes of infertility.

Some other symptoms of high testosterone in women are:

  • Excess body and facial hair
  • Voice like men
  • Menstrual irregularities
  • Delayed or absence of menses
  • Excessive oily skin
  • Recurrent acne
  • Low sexual desire
  • Dryness of vagina
  • Painful coitus
  • Hairfall
  • Sweet cravings
  • Enlarged clitoris
  • Weight gain
  • Issues in getting pregnant

However, abnormal testosterone levels in the blood do not always indicate medical problems. As men grow older and exercise excessively, their testosterone levels begin to decrease.

How Frequently should you take this Test?

How Frequently should you take this Test?

In Males

Like most chronic diseases, low-level testosterone treatment takes time. As a result, the doctor will most likely repeat the test to read the testosterone values after three months to monitor the levels and keep an eye on the treatment strategy.

This three-month check-up will reveal whether the amount has increased, decreased or remained the same. The doctor repeats this test annually if the testosterone levels are in a normal range in the previous report but were erratic before.

In Females

In females, if the doctor suspects PCOS or increased testosterone, he/she may ask for a testosterone test. The test is repeated every three months to monitor levels of testosterone range after treatment.

Test Preparation

Test Preparation

What to Expect When You Take the Test

Before the Test

Usually, an Testosterone test doesn't need any special prep. If you're taking this test with another one, your doctor might tell you to avoid eating certain foods. It's always a good idea to talk to your doctor before the test to get the best results.

During the Test

The phlebotomist will take a blood sample from a vein in your arm. Here's what you can expect:

  • The spot where they're going to insert the needle is cleaned with an antiseptic.
  • A band/tourniquet will be wrapped around your arm to make your veins stand out more.
  • A needle will be guided into the vein to draw the blood sample. This might take a couple of seconds, and you might feel a quick pinch.
  • The sample blood will be collected in a vial/small tube with your details on it.

After the Test

After the Blood is Collected:

  • A bandage will be applied over the puncture spot to stop the bleeding.
  • You might develop a bruise, but feeling dizzy is rare. You might be asked to sit down for a bit.
  • If you notice any bleeding, pain, or a rash at the puncture site, let your doctor know.
Parameters

Parameters

The testosterone test includes only one parameter. The testosterone test values give an idea about the decrease and increase in the testosterone values.

Ranges

Ranges

The normal testosterone range depends on both gender and age.

Normal testosterone results in adult men

Age

Results

20 to 49

4.56 - 28.2 nmol/L

50 and older

2.49 - 21.6 nmol/L

Normal testosterone results in adult women with normal menstrual cycle- 0.198- 2.67 nmol/L

The normal values and reference ranges of the test may vary from lab to lab. Please refer to the ranges mentioned in the report and consult a doctor to understand the interpretation of lab reports.

Test Result Interpretation

Test Result Interpretation

Factors like exercise and old age affect testosterone levels in men. Test interpretation needs consideration of these variable factors. Various sexual dysfunctions are caused by increased or decreased amounts of testosterone in the blood. Abnormal levels of testosterone in males could be a sign to start making lifestyle changes.

Decreased level of testosterone means some underlying cause that is affecting testosterone levels.

Hypogonadism

It is a condition in which there is decreased functional activity of the gonads leading to a decrease in hormone production like testosterone, estradiol, progesterone, etc.

Testicular failure

Despite adequate hormones, the testicles fail to produce sperm in this disease. Testicular failure occurs due to increased luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels. In this disease, values are low in the testosterone chart.

Testicular cancer

Testicular cancer strikes at an early age and is treatable. A low amount of testosterone is detected during the diagnosis and screening of testicular cancer.

Increased testosterone levels mean tumours producing increased levels of testosterone.

In young boys

Increased testosterone report values are seen in boys before puberty due to testicular or adrenal gland cancer. However, added investigations are required to determine the reason for elevated testosterone levels.

In adult males

Excessive testosterone supplements are among the causes of increased values in testosterone reports of adult males.

Abnormal levels of testosterone in females are a sign of many diseases. Decreased testosterone values could be due to:

Primary or secondary ovarian failure

The ovaries stop functioning properly and stop releasing oestrogen or testosterone, resulting in a low testosterone level in the body.

Increased testosterone values could be due to:

Congenital adrenal hyperplasia (CAD)

CAD is a hereditary condition that affects the adrenal glands. The adrenal glands cause an increase in testosterone production. CAD results in ambiguous genitalia, enlarged clitoris or genitals that resemble a male infant.

Prepubertal girls

Girls go through a stage similar to males before puberty. The testosterone chart shows higher values in this period.

Adrenal tumours

Adrenal tumours are benign tumours that grow on the adrenal glands. The tumour produces increased levels of testosterone. The adrenal tumours present with excessive facial hair, body hair and a larger clitoris.

Ovarian tumours

The level of hormones secreted in the body can also be affected by ovarian cancer. Ovarian tumours result in an increase of testosterone in females.

Polycystic ovarian syndrome

PCOS and hyperandrogenism are linked. One of the possible causes of the polycystic ovarian syndrome is elevated androgens. PCOS presents with symptoms like hirsutism, acne, menstrual irregularities, insulin resistance and in many cases, obesity. In this condition, the testosterone report shows higher values.

Price/Cost

Price/Cost

Depending on the city and the lab, the cost of a Testosterone test can differ. Generally, the price for a Testosterone test ranges from INR 315 to INR 825. Here's a detailed breakdown of the average expenses for a Testosterone test in several key cities in India:

City

Min Price

Average Price

Max Price

Testosterone Test Cost in Bengaluru

315

570

825

Testosterone Test Cost in Chennai

315

570

825

Testosterone Test Cost in Delhi

315

570

825

Testosterone Test Cost in Hyderabad

315

570

825

Testosterone Test Cost in Kolkata

315

570

825

Testosterone Test Cost in Lucknow

315

570

825

Testosterone Test Cost in Mumbai

315

570

825

Testosterone Test Cost in Nagpur

315

570

825

Testosterone Test Cost in Patna

315

570

825

Testosterone Test Cost in Pune

315

570

825



Risk and Limitations

Risk and Limitations

The Testosterone test is a commonly done blood test with rare risks of complications. See your doctor if you notice:

  • Persistent bleeding at the site of needle-insertion.
  • The skin where the needle went in becomes red, swollen, or hurts.

Limitations of the test

  • The accuracy of the test could be affected due to equipment or human mistakes.
  • Misinterpretation of the markers leading to inaccurate report.

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People Also Ask

Does masturbating reduce testosterone?

Masturbation does not have any correlation with lowering testosterone. However, it may have some short-term effects on the hormone, but it will not affect your overall sexual health.

Is 400 a good testosterone level?

Yes, 400 ng/dl is a good testosterone level. According to studies, the normal testosterone range in young males is between 249 - 836ng/dL. In adult males above 50 years, normal testosterone levels are between 193 - 740 ng/dL. The value of 400ng/dL is a healthy range for males and females.

How can I test my testosterone levels at home?

Testosterone values can now be checked at home as well. A home test kit will help you know the exact values of testosterone in your blood sample. This kit comes with an instruction manual. You need to follow instructions carefully. The blood sample is sent to the laboratory for analysis. The results will be sent to you by the laboratory. You can also avail home sample collection done by experienced phlebotomists from the comfort of your home from PharmEasy Labs.

How do you fix low testosterone?

The doctor will start testosterone replacement therapy depending on the values in the testosterone reports. Along with the medications, certain lifestyle modifications and dietary changes can help you boost testosterone values. Adopting a nutritious diet, sleeping for eight hours and avoiding alcohol consumption can naturally improve testosterone values.

How much does a Testosterone test cost?

It's essential to note that the Testosterone test cost may differ significantly, ranging from Rs 315 to Rs 825, based on factors like your location, choice of diagnostic lab and potential discounts.

Have any doubts? Ask us.

Ask us anything about the Testosterone Test to understand it better

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