Anti Nuclear Antibody (ANA-Elisa) Test
Booked 56 timesAlso known as:
Antinuclear antibody test, ANA test
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Overview
The antinuclear antibodies bind to the contents of the nucleus of the cell. In some cases, the body produces antinuclear antibodies, which are antibodies against human antigens.
There are many subtypes of ANAs such as:
- Anti-Ro antibodies
- Anti-La antibodies
- Anti-Sm antibodies
- Anti-RNP antibodies
- Anti-Scl-70 antibodies
- Anti-dsDNA antibodies
- Anti-histone antibodies
- Anti-centromere antibodies
- Anti-sp-100 antibodies
These antibody subtypes bind to different proteins or protein complexes within the nucleus.
The ANA test detects the antinuclear autoantibodies present in a patient's blood. The standard tests used for detecting and quantifying ANAs are enzyme-linked immunosorbent assay (ELISA). The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical biochemistry assay.
A doctor may recommend an ANA (Elisa) Test when you have symptoms of autoimmune diseases like severe fever, difficulty in breathing, vision difficulty, hair loss, weakness, abnormal growth, severe abdominal pain, and many more.
When the test results are positive and show a high positive presence of antinuclear antibodies in your blood or serum. In that case, you may have severe autoimmune-related disorders and diseases like Sjögren's syndrome, Autoimmune hepatitis, polyarthritis nodosa, rheumatoid arthritis, dermatomyositis and several other health complications.
Study results among US adults aged 12 and older indicate that 13.8% have ANA-positivity, compared with 12.3% in India.
Other Names for ANA (Elisa) Test
- Fluorescent Antinuclear Antibody Test
- Antinuclear Antibody Screen Test
- FANA Test
- ANA IgG Screen Test
- Antinuclear Ab Test
What does the test measure and who is this prescribed for?
The ANA (Elisa) Test measures the presence of antinuclear antibodies in the blood. ANA test results that are positive or abnormal may affect the blood or organs in various ways.
Your doctor may recommend an ANA (Elisa) test if you have symptoms that include or are related to different parts of the body.
Some symptoms are:
- Fatigue
- Recurring or persistent fever
- Difficulties or shortness in breathing
- Skin rashes
- Sores on the skin
- Abdominal discomfort, cramps or severe pain
- Bleeding in the rectus
- Diarrhoea (continuous passing of watery stool)
- Difficulty or permanent loss of hearing
- Itching, stiffness or redness in the eyes
- Vision complications like blurred vision or permanent loss of vision
- Running nose
- Tiredness or weakness
- Hair loss
- Numbness and tingling in hands or feet
The doctor may suggest an ANA (Elisa) Test if the person has already detected diseases or disorders like:
- Raynaud's syndrome- A condition that makes your fingers and toes turn blue and feel cold.
- Thyroid diseases (due to dysfunction or abnormal thyroid gland).
- Lung diseases like pulmonary fibrosis.
- Inflammatory bowel diseases.
- Liver diseases like autoimmune hepatitis, liver cirrhosis.
- A cancerous tumour.
- Infections caused by viruses.
Antinuclear antibodies can be detected in 20% of healthy people despite not having an autoimmune disease.
A false-positive result is more likely in the case of:
- An infection such as mononucleosis or tuberculosis.
- Anti-seizure medicine or blood pressure medication.
- A woman aged 65 or older.
If the test results of the ANA (Elisa) Test show high positivity in the blood, the diagnosis of any autoimmune diseases has to be ruled out in that case. You need further treatment and monitoring by a consultant physician, rheumatologist, or immunologist. A doctor may also recommend you a repetitive ANA Test for three months.
A doctor uses nonsteroidal anti-inflammatory drugs like ibuprofen and naproxen in treating a patient with a high positive ANA in blood. Several medications are available for Lupus patients, including hydroxychloroquine or chloroquine, to reduce inflammation.
Sample Type
A simple blood sample or blood serum is needed to endure an antinuclear antibody test. This test does not require any specific preparation.
Test Preparation
The ANA test needs a blood sample. A simple procedure for blood withdrawal is by wrapping an elastic band around the arm and ceasing the area by wiping alcohol. Then, the professional inserts a small needle into the vein to pull out a sample of blood. Blood is collected in a vial and is sent to the lab for analysis. No other specific preparation is needed for the test.
Test inclusion: What are the parameters included in the test?
Antinuclear antibodies are autoantibodies which might affect the nucleus of healthy cells in your body. The Antinuclear Antibody Test measures the presence of antinuclear antibodies in your blood.
It also helps in confirmation of the diagnosis of some autoimmune diseases. The high positive presence of ANA in the blood can cause severe health complications.
Ranges
The positive presence of antinuclear antibodies can affect the patient's health. At the same time, negative presence does not affect the patient's health—the normal range for detecting antinuclear antibodies.
- The average or negative presence of ANA titer is measured as less than or equal to 1:40.
- The average significant results of ANA titer are measured as 1:80 or lower.
- The strongly positive ANA titer is measured between 1:160.
The normal values, parameters, and reference ranges of the test may vary from lab to lab. Please refer to the ranges mentioned in the report and consult a doctor to understand the interpretation of lab reports.
Test Result Interpretation
There are mild to severe health problems due to the high positive presence of antinuclear antibodies in the blood. At the same time, the negative value does not affect anyone's health.
High positive presence of ANA (Elisa)
- Sjögren's syndrome: It is a type of autoimmune disease and chronic disorder that causes insufficient moisture production in certain body glands, including the saliva-producing and tear-producing glands. In addition to Sjögren's syndrome, there are other related diseases, such as rheumatoid arthritis and lupus.
- Scleroderma: A chronic but rare autoimmune disease in which normal tissue is replaced with thick tissue with extra collagen. It most often affects the skin, and scleroderma also can affect many other parts of the body like joints, bones, etc.
- Rheumatoid arthritis: A form of autoimmune disease in which your immune system attacks healthy cells in your body, causing swelling (inflammation) in the affected parts of the body. RA mainly affects many joints, including those in the hands and feet.
- Dermatomyositis: An uncommon inflammatory disease in which muscle weakness and a distinctive skin rash on the body can develop. The condition can affect adults in their late 40s or 60s and children from 5 to 15 years of age.
- Autoimmune hepatitis: A chronic liver inflammation that occurs when your body's immune system turns against liver cells. The cause of autoimmune hepatitis is still unknown, but genetic and environmental factors can affect the disease. It can often be controlled if diagnosed or treated early.
- Polyarteritis Nodosa (PAN): It is a rare disease of autoimmune disease that results from blood vessel inflammation (vasculitis), causing injury to organ systems. The PAN most commonly affects the area of the nerves, intestinal tract, heart and joints of the body.
Negative presence of ANA test (Elisa)
The negative or typical test results of the ANA (Elisa) test mean that you don't suspect an autoimmune disorder or other health condition related to ANA.
Risk Assessment
Autoimmune diseases, Polymyositis, Drug-induced lupus
Price/Cost
The price of an ANA Test can differ based on the city and the laboratory. Typically, an ANA test falls within the range of INR 375 to INR 935. For a better understanding, here is the average cost of an ANA test in some major cities in India:
City | Min Price | Average Price | Max Price |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 | |
375 | 655 | 935 |
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Frequently Asked Questions
What diseases can cause a positive ANA?
Several health conditions usually cause a positive ANA test, including Scleroderma, Systemic Lupus erythematosus, Rheumatoid arthritis, Sjögren's syndrome, Polymyositis, etc.
Is a positive ANA test terrible?
When detected at high positive ANA, the result does not indicate that a patient has or will develop autoimmune disease in the absence of symptoms or physical findings. While having high positive ANA test results with symptoms of autoimmune diseases, the condition can cause severe health complications.
What is an ANA test used to diagnose?
An ANA test is used to diagnose autoimmune disorders like lupus and autoimmune hepatitis or detect the presence of antinuclear antibodies in the blood.
What happens after a positive ANA test?
A positive result on an ANA test may help a doctor to diagnose an autoimmune disorder. After the results appear as a positive presence of ANA in the test, the patient will be recommended for another examination or should start treatment for a particular autoimmune condition immediately after the diagnosis.
Can stress cause positive ANA?
Stress may cause autoimmune diseases, such as lupus or rheumatoid arthritis, based on a study that found a higher incidence of autoimmune diseases among people with stress-related disorders. A link between stress and ANA reactivity was found among patients with connective tissue disease (CTD).
Can low vitamin D cause positive ANA?
Vitamin D deficiency may contribute to immune dysfunction, resulting in the production of antibodies, particularly antinuclear antibodies (ANA). A linear relationship wherein patients with severe vitamin D deficiency demonstrated 2.99 increased odds of receiving a positive ANA test.
What drugs cause positive ANA?
Drugs like Isoniazid, Methyldopa, Quinidine, Minocycline, Sulfadiazine and Hydralazine are associated medications with positive antinuclear antibodies in the blood. The condition is then called drug-induced auto autoimmune disease.
How frequently should you take this test?
Your doctor may recommend you repeat an ANA Test every three months if you find recurrent symptoms or your previous test results show a positive presence of antinuclear antibody in your blood. If previous ANA test results are negative, you can repeat your test on an annual or recurrent symptom basis.
What is the price of an ANA test?
The ANA test price is not standardized, as it can vary from Rs 375 to Rs 935 based on factors such as your location, the diagnostic lab you select and any discounts that may apply.