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About the test
What is this test done for?
Rh antibody titre is a blood test used for detecting antibodies against Rh factor present on red blood cells of our body.
Why is it Done?

This is a special blood test that evaluates presence of antibodies that are prepared against Rh (Rhesus) factor, which is present on RBCs. This causes destruction of RBCs and causes hemolytic anemia.

The test is done for identifying the cause of hemolytic anemia, like

  • Auto immune diseases like SLE (systemic lupus erythematosus)
  • Malignant diseases like lymphoma or chronic lympocytic leukemia
  • Infection like mononucleosis and mycoplasma pneumonia
  • Hemolytic disease of the new born (HDN)
When should it be performed?

This test is performed if

  • A mother, who is Rh negative and has previous exposure to Rh factor, and in current pregnancy has Rh positive baby. This results in formation of antibodies against the baby’s RBCs and causes hemolytic disease of the new born. Thus, in pregnancy, this test is performed to rule out possibility of hemolytic complications in mother and baby.
  • A patient presents with symptoms of hemolytic anemia
    • Jaundice (yellowish color of eyes, dark yellow urine)
    • Headache and lightheadedness
    • Pale skin
    • Shortness of breath
    • Lowered blood pressure
    • Shock
    • Fever with chills
    • Enlarged spleen
    • Sore tongue
    • Back pain
    • Blood in urine
How is it done
A blood sample will be collected from a vein in your arm. No special preparations like fasting required.

The test result is normal, if the test is negative. It means

  • No antibodies are attached to RBCs
  • Absence of hemolytic anemia

The test results are abnormal, if the test is positive. Stronger the reaction means greater the amount of antibodies are present. It means

  • Antibodies are present against RBCs and is causing hemolysis
  • Blood transfusion reaction
  • High possibility of hemolytic disease of the new born (baby-mother blood group incompatibility)
  • Auto immune reaction or auto immune disease like systemic lupus erythematosus
  • Infections such as mononucleosis or mycoplasma pneumonia
  • Adverse reaction to penicillin
Other Tests
  • CBC
    • Reduced amount of RBCs and drop in hemoglobin suggest anemia
  • RBC antibody screen or Indirect Coombs Test
    • It is a specialized blood test carried out to evaluate the presence of antibodies against RBCs
    • Presence of antibodies indicate hemolysis
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