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Anti D Titre

About the test
What is this test done for?
Anti D titer stands for direct antiglobulin test, which is commonly known as direct Coomb’s test. This test is done for checking the presence of antibodies against RBCs (red blood cells).
Why is it Done?
This is a special blood test that checks for antibodies prepared against Rh (Rhesus) factor present on RBCs in the blood. This results in the destruction of RBCs, causing a condition called as hemolytic anemia. The test is done for identifying the cause of hemolytic anemia
  • Auto immune disorder such as systemic lupus erythematosus
  • Malignant disease like lymphoma or chronic lympocytic leukemia
  • Infection such as mononucleosis and mycoplasma pneumonia
  • Hemolytic disease of the new born
When should it be performed?
This test is performed when
  • A patient presents with symptoms of hemolytic anemia
    • Jaundice (yellowish color of eyes, dark yellow urine)
    • Headache and lightheadedness
    • Pale skin
    • Shortness of breath
    • Lowered blood pressure
    • Shock
    • Fever with chills
    • Enlarged spleen
    • Sore tongue
    • Back pain
    • Blood in urine
  • A mother, who is Rh negative and has previous exposure to Rh factor, and in the current pregnancy has Rh positive baby. This results in formation of antibodies against the baby’s RBCs and causes hemolytic disease of the new born. Thus, this test is performed in pregnancy to rule out hemolytic complications
How is it done
A blood sample will be collected from a vein in your arm. No special precautions like fasting required.
Results
The test result is normal if the test is negative. It means
  • No antibodies are attached to RBCs
  • Absence of hemolytic anemia
The test results are abnormal if the test is positive. Stronger the reaction means greater the amount of antibodies present. It means
  •  Antibodies are present against RBCs and is causing hemolysis
  • Blood transfusion reaction
  • High possibility of hemolytic disease of the new born (baby-mother blood group incompatibility)
  • Autoimmune reaction or autoimmune disease like systemic lupus erythematosus
  • Infections such as mononucleosis or mycoplasma pneumonia
  • Adverse reaction to penicillin
Other Tests
  • CBC
    • Reduced amount of RBCs and drop in hemoglobin suggests anemia
  • RBC antibody screen or Indirect Coombs Test
  • It is a specialized blood test carried out to evaluate the presence of antibodies against RBCs
  • Presence of antibodies indicate hemolysis
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